Establishment of an efficient primary callus induction for embryogenic potential of Coffea arabica

Coffea arabica L. is a species of coffee that contribute for more than seventy percent world production. Various attempts have been made to obtain large quantities planting material through propagation in vitro somatic embryogenesis technology. The objective this experiment was evaluate the effect different plant growth regulators (PGRs) on callus induction (indirect embryogenesis) AS2K clone Arabica coffee. Mother plants were established experimental field Indonesian Coffee and Cocoa Research Institute at Andung Sari, Bondowoso, East Java, Indonesia (-7˚55'' ' S, 113˚41'' E) an altitude 1380,1 m dpl. Leaf explants cultured half-strength Murashige Skoog (MS) medium supplemented with various concentration (1.0, 2.0, 3.0 mg/L) 2,4-D thidiazuron combination 1.0 mg/L BAP. All experiments organized completely random design (CDR) repeated three times, each using minimum seven replicates (a total 21 per treatment). morphologycal histological analysis types observed. percentage formation recorded every two weeks until eight weeks. highest (almost 60%) containing 1 dan Morphological studies prove has friable embryogenic texture begins develop stages embryo formation, starting globular, heart, torpedo cotyledonary phases.